investigators had proteins extracted from mouse brain tissue and used them to react
Description
In this study, investigators had proteins extracted from mouse brain tissue and used them to react with sera from patients with CIDP by western blotting to determine the presence of common bands. The bands reported as positive were then characterized by mass spectrometry and confirmed for reactivity with the patient sera utilizing using enzyme-linked immunosorbent assay and western blotting. In further testing, the serum immunoglobulin G (IgG) and IgM antibodies' reactivity to recombinant DLAT was then confirmed by cell-based and tissue-based indirect immunofluorescence assays. Authors also analyzed the clinical characteristics of patients with candidate autoantibody-positive CIDP and their association with other neurologic diseases.
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